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The alkaline protease producing bacteria isolated from soil and identified as Bacillus subtilis. The optimum medium for alkaline protease production from the microorganism was as follows; soluble starch, 1.5% ; proteose peptone, 0.5% ; K₂HPO₄, 0.1% ; MgSO₄, 7H₂O, 0.02% and sodium carbonate, 1.0%. The optimum temperature for alkaline protease production was 35℃, and the initial pH of medium was pH 10.5. The alkaline protease activity was about 2,300 U per ㎖ of culture broth by Casein-Folin Method. A 9.2 fold purification of alkaline protease was obtained from culture broth. The recovery was 14% and purified enzyme was identified as single band, and its molecular weight was about 19,000. The optimum temperature for enzyme reaction was 70℃, and optimum pH was 12. The activity of purified enzyme was inhibited by metal ion (Fe^(++)), and Phenylmethylsulfonyl Fluoride, a serine protease inhibitor.
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